Ovicidal efficacy of the alcoholic extract of Moringa oleifera Lam seeds on Fasciola hepatica

Abstract

The objective of this study was to determine the ovicidal effect of alcoholic and aqueous extracts of Moringa oleifera seeds on Fasciola hepatica eggs. M. oleifera seeds were ground and immersed in methanol (1:10) at room temperature for 2 days with discontinuous rotation and then filtered through filter paper. The concentration of the methanolic extract was carried out under reduced pressure using a rotary evaporator at 4°C. The concentrated methanolic extract was kept at 40°C until use. For use, the methanolic extract was dissolved in distilled water with the addition of a few drops of Tween-80. For the aqueous extract, 15 g of M. oleifera seeds were weighed, which were crushed, and 1 L of distilled water was added. Then, they were boiled for 3 minutes, with subsequent cooling and filtration. Then, the decoction was concentrated under vacuum at 40 °C, frozen in an alcohol spin bath, before being subjected to desiccation, under the same conditions as the methanolic extract. The results show that the best ovicidal effect was obtained with the methanolic extract at a concentration of 20 mg/ml, with a 100% inhibition of viability and hatchability on F. hepatica eggs. It is concluded that the methanolic extract of M. oleifera was effective in inhibiting the viability and hatching of F. hepatica eggs at a concentration of 20 mg/mL.